Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis

Author:

Hu Tao1,Niu Yunfeng2,Fu Jianfeng1,Dong Zhiming2,He Dongwei2,Liu Junfeng3

Affiliation:

1. Department of Anesthesiology, The Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China

2. Laboratory of Pathology, Hebei Cancer Institute, The Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China

3. Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University , Shijiazhuang , Hebei , China

Abstract

Abstract Multiple studies have indicated that long non-coding RNAs are aberrantly expressed in cancers and are pivotal in developing various tumors. No studies have investigated the expression and function of long non-coding antisense RNA PCNA-AS1 in esophageal squamous cell carcinoma (ESCC). In this study, the expression of PCNA-AS1 was identified by qRT–PCR. Cell function assays were used to explore the potential effect of PCNA-AS1 on ESCC progression. A prediction website was utilized to discover the relationships among PCNA-AS1, miR-2467-3p and proliferating cell nuclear antigen (PCNA). Dual luciferase reporter gene and RNA immunoprecipitation (RIP) assays were executed to verify the binding activity between PCNA-AS1, miR-2467-3p and PCNA. As a result, PCNA-AS1 was highly expressed in ESCC and was associated with patient prognosis. PCNA-AS1 overexpression strongly contributed to ESCC cell proliferation, invasion and migration. PCNA-AS1 and PCNA were positively correlated in ESCC. Bioinformatics analysis, RIP and luciferase reporter gene assays revealed that PCNA-AS1 could act as a competitive endogenous RNA to sponge miR-2467-3p, thus upregulating PCNA. In conclusion, the current outcome demonstrates that PCNA-AS1 may be a star molecule in the treatment of ESCC.

Publisher

Walter de Gruyter GmbH

Subject

General Medicine

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