Evaluation of two fully automated immunoassay based tests for the measurement of 1α,25-dihydroxyvitamin D in human serum and comparison with LC-MS/MS

Abstract

AbstractBackground:1α,25-Dihydroxyvitamin D [1,25(OH)Methods:In the presented study, we evaluated the performance of two immunoassays from DiaSorin and from Immunodiagnostic Systems (IDS) which combine fully automated extraction and measurement of 1,25(OH)Results:Total imprecision was 5.2% or less for the DiaSorin test but reached 20.1% for the IDS iSYS test. 1,25(OH)Conclusions:Due to its high sensitivity, low imprecision, broad measurement range, and good agreement with 1,25(OH)