Identification Of Liver Mitochondria Tumor Associated Antigens Of The Mice Exposed To N, Nitrosodibutylamine: A Promising Strategy In Development Of Anticancer Treatment

Abstract

Background: N-Nitrosodibutylamine possess the ability to induce bladder tumor as well as in the liver, and oesophagus when it is administered in the body.Exposure to N-Nitrosodibutylamine can happen by different modes such as by ingestion,inhalation and dermal contact.Methods:In the present investigation an attempt has been done to identify ,isolate as well to purify the tumor associated antigens (TAA) from the liver mitochondria of the mice when exposed to N-Nitrosodibutylamine.Results:It was found that mitochondrial membrane protein of N-Nitrosodibutylamine-exposed animals exhibited differential expression of protein when compared with the control animals. A low molecular weight (~14 kDa) protein was found to be over expressed significantly on liver mitochondrial membrane upon N-Nitrosodibutylamine exposure in mice as compared with the normal control and identified as TAA,showing the sign that some of the proteins could be used as TAA for further study.Conclusion: These identification of TAAs will provide the basis for the use of mitochondria as markers for the early detection for the treatment of liver cancer.