EXPRESSION OF H/ACA snoRNA IN CELL LINES WITH CHROMOSOMAL ABNORMALITIES AFTER IRRADIATION

Abstract

The H/ACA snoRNA family is involved in pseudouridine biogenesis. It prevents genetic changes in cells and makes them more stable due to ribosomal RNA characteristics. Therefore, the study of H/ACA snoRNA expression in cell lines with chromosomal disorders after irradiation is of particular interest. The purpose of the study is to analyze the effect of chromosomal disorders on H/ACA snoRNA expression in radioresistant K562 and radiosensitive HL-60 cell lines after radiation exposure. Materials and Methods. K562 and HL-60 cell lines were exposed to radiation (4 Gy). H/ACA snoRNA expression was analyzed by NGS sequencing (1, 4, and 24 hours after irradiation). Results. The authors revealed differences in H/ACA snoRNA expression by chromosomes in the studied cell lines, as well as the impact of chromosomal abnormalities on H/ACA snoRNA expression after radiation exposure. Changes in the copy number of normal chromosomes lead to minor changes in H/ACA snoRNA expression. Marker chromosomes disrupt H/ACA snoRNA expression. Thus, is becomes impossible to use H/ACA snoRNAs located in abnormal chromosomes as radioresistance markers. Moreover, marker chromosomes decrease the number of H/ACA snoRNAs expressed in K562, despite the greater amount of genetic material.