Characterization and comparison of recombinant honeybee chymotrypsin-like protease (HCLPase) expressed in Escherichia coli and insect cells-Reference-Cited by-同舟云学术

Characterization and comparison of recombinant honeybee chymotrypsin-like protease (HCLPase) expressed in Escherichia coli and insect cells

Published:2017-07-03 Issue:7 Volume:81 Page:1401-1404
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Matsuoka Takuma¹,Kawashima Takuji¹,Nakamura Tadashi¹,Yabe Tomio²³

Affiliation:

1. Akitayahonten Co., Ltd., Gifu, Japan

2. Department of Applied Life Science, Gifu University, Gifu, Japan

3. Center for Highly Advanced Integration of Nano and Life Sciences, Gifu University (G-CHAIN), Gifu, Japan

Abstract

Abstract We previously found a novel chymotrypsin-like protease in honeybee, designated as HCLPase. The recombinant enzyme expressed in insect cells was produced and compared to that in Escherichia coli. Both enzymes showed equivalent molecular size and specificity. However, HCLPase produced in insect cells showed higher specific activity. The C-terminal cleavage sites of HCLPase were phenylalanine, leucine, and tyrosine residues.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/81/7/1401/36824014/bbb1401.pdf

Reference12 articles.

1. Significance of royal jelly for honeybee queen;Matsuka;Honeybee Sci,1998

2. Royalactin induces queen differentiation in honeybees;Kamakura;Nature,2011

3. The evolution of endopeptidases—XII. The proteolytic enzymes of the honeybee (Apis Mellifica L.): purification and characterization of endopeptidases in the midgut of adult workers and comparative studies on the endopeptidase-pattern of different caste and on different ontogenetic stage;Giebel;Comp Biochem Physiol B Comp Biochem,1971

4. The midgut endopeptidases of the honey bee (Apis mellifica): comparison of the enzymes in different ontogenetic stages;Dahlman;Insect Biochem,1987

Cited by 3 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Recombinant Chymotrypsin-like Peptidase from Tenebrio molitor with a Non-Canonical Substrate-Binding Site;Applied Biochemistry and Microbiology;2024-05-16

2. Raman spectrum combined with deep learning for precise recognition of Carbapenem-resistant Enterobacteriaceae;Analytical and Bioanalytical Chemistry;2024-02-21

3. Detection and pH-Thermal Characterization of Proteinases Exclusive of Honeybee Worker-Fate Larvae (Apis mellifera L.);International Journal of Molecular Sciences;2022-12-08