Transcriptomic features associated with energy production in the muscles of Pacific bluefin tuna and Pacific cod

Author:

Shibata Mami1,Mekuchi Miyuki2,Mori Kazuki3,Muta Shigeru4,Chowdhury Vishwajit Sur5,Nakamura Yoji2,Ojima Nobuhiko2,Saitoh Kenji2,Kobayashi Takanori6,Wada Tokio6,Inouye Kiyoshi7,Kuhara Satoru4,Tashiro Kosuke4

Affiliation:

1. Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan

2. Research Center for Aquatic Genomics, National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Japan

3. Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan

4. Laboratory of Molecular Gene Techniques, Division of Molecular Biosciences, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan

5. Division for Experimental Natural Science, Faculty of Arts and Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan

6. Fisheries Research Agency, Yokohama, Japan

7. Japan Fisheries Science and Technology Association, Tokyo, Japan

Abstract

Abstract Bluefin tuna are high-performance swimmers and top predators in the open ocean. Their swimming is grounded by unique features including an exceptional glycolytic potential in white muscle, which is supported by high enzymatic activities. Here we performed high-throughput RNA sequencing (RNA-Seq) in muscles of the Pacific bluefin tuna (Thunnus orientalis) and Pacific cod (Gadus macrocephalus) and conducted a comparative transcriptomic analysis of genes related to energy production. We found that the total expression of glycolytic genes was much higher in the white muscle of tuna than in the other muscles, and that the expression of only six genes for glycolytic enzymes accounted for 83.4% of the total. These expression patterns were in good agreement with the patterns of enzyme activity previously reported. The findings suggest that the mRNA expression of glycolytic genes may contribute directly to the enzymatic activities in the muscles of tuna.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

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