Direct visualization of replication dynamics in early zebrafish embryos-Reference-Cited by-同舟云学术

Direct visualization of replication dynamics in early zebrafish embryos

Published:2016-05-03 Issue:5 Volume:80 Page:945-948
ISSN:0916-8451
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Kuriya Kenji¹,Higashiyama Eriko¹,Avşar-Ban Eriko²,Okochi Nanami¹,Hattori Kaede¹,Ogata Shin¹,Takebayashi Shin-ichiro³,Ogata Masato³,Tamaru Yutaka²,Okumura Katsuzumi¹

Affiliation:

1. Laboratory of Molecular & Cellular Biology, Department of Life Sciences, Graduate School of Bioresources, Mie University, Tsu, Japan

2. Laboratory for the Utilization of Aquatic Bioresources, Department of Life Sciences, Graduate School of Bioresources, Mie University, Tsu, Japan

3. Department of Biochemistry and Proteomics, Graduate School of Medicine, Mie University, Tsu, Japan

Abstract

Abstract We analyzed DNA replication in early zebrafish embryos. The replicating DNA of whole embryos was labeled with the thymidine analog 5-ethynyl-2ʹ-deoxyuridine (EdU), and spatial regulation of replication sites was visualized in single embryo-derived cells. The results unveiled uncharacterized replication dynamics during zebrafish early embryogenesis.

Funder

Kieikai Research Foundation

The Public Foundation of Elizabeth Arnold-Fuji

Yamazaki Spice Promotion Foundation

Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/article-pdf/80/5/945/36828308/bbb0945.pdf

Reference19 articles.