Direct bioethanol production from brown macroalgae by co-culture of two engineered Saccharomyces cerevisiae strains

Author:

Sasaki Yusuke123,Takagi Toshiyuki234,Motone Keisuke234,Shibata Toshiyuki35,Kuroda Kouichi23,Ueda Mitsuyoshi23

Affiliation:

1. Graduate School of Advanced Integrated Studies in Human Survivability, Kyoto University , Kyoto, Japan

2. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University , Kyoto, Japan

3. CREST, JST , Kyoto, Japan

4. Japan Society for the Promotion of Science , Kyoto, Japan

5. Department of Life Sciences, Graduate School of Bioresources, Mie University , Mie, Japan

Abstract

ABSTRACT A co-culture platform for bioethanol production from brown macroalgae was developed, consisting of two types of engineered Saccharomyces cerevisiae strains; alginate- and mannitol-assimilating yeast (AM1), and cellulase-displaying yeast (CDY). When the 5% (w/v) brown macroalgae Ecklonia kurome was used as the sole carbon source for this system, 2.1 g/L of ethanol was produced, along with simultaneous consumption of alginate, mannitol, and glucans.

Funder

JST, CREST

Grant-in-Aid for JSPS Fellows

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

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