Polysaccharide hydrolase of the hadal zone amphipods Hirondellea gigas

Author:

Kobayashi Hideki1,Nagahama Takahiko2,Arai Wataru1,Sasagawa Yohei3,Umeda Mana3,Hayashi Tetsutaro3,Nikaido Itoshi3,Watanabe Hiromi1,Oguri Kazumasa1,Kitazato Hiroshi1,Fujioka Kantaro4,Kido Yukari1,Takami Hideto1

Affiliation:

1. Japan Agency for Marine-Earth Science and Technology (JAMSTEC) , Yokosuka, Japan

2. Department of Foods and Human Nutrition, Notre Dame Seishin University , Okayama, Japan

3. Bioinformatics Research Unit, Advanced Center for Computing and Communication, RIKEN , Saitama, Japan

4. Faculty of Engineering, Kanagawa University , Yokohama Chity, Japan

Abstract

Abstract Hirondellea species are common inhabitants in the hadal region deeper than 7,000 m. We found that Hirondellea gigas thrived in the Challenger Deep possessed polysaccharide hydrolases as digestive enzymes. To obtain various enzymes of other H. gigas, we captured amphipods from the Japan Trench, and Izu-Ogasawara (Bonin) Trench. A phylogenetic analysis based on the cytochrome oxidase I gene showed close relationships among amphipods, despite the geographic distance between the localities. However, several differences in enzymatic properties were observed in these H. gigas specimens. We also carried out RNA sequencing of H. gigas from the Izu-Ogasawara Trench. The cellulase gene of H. gigas was highly homologous to cellobiohydrolase of Glucosyl Hydrolase family 7 (GH7). On the other hand, enzymatic properties of H. gigas’s cellulase were different from those of typical GH7 cellobiohydrolase. Thus, these results indicate that hadal-zone amphipod can be good candidates as the new enzyme resource.

Funder

Japan Society for the Promotion of Science

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

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