Validation of a Toll-like Receptor (TLR)2/TLR1 Activation Assay for Biological Standardization of Arthrospira / Limnospira Immune-Enhancing Potency

Author:

Haron Mona H.1,Zhang Jin1,Chittiboyina Amar G.1,Khan Ikhlas A.12,Pugh Nirmal D.

Affiliation:

1. National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, MS, USA

2. Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, USA

Funder

National Institutes of Health

USDA, Agricultural Research Service Specific Cooperative Agreement

Publisher

Informa UK Limited

Subject

Pharmacology (medical),Nutrition and Dietetics,Food Science

Reference15 articles.

1. Chesher D. 2008. Evaluating assay precision. Clin Biochem Rev. 29(Suppl 1):S23–S26.

2. FDA. 2023. FDA Revised Botanical Drug Development Guidance for Industry [accessed April 4 2023]. https://www.fda.gov/media/93113/download.

3. HEK-Blue TM hTLR2-TLR1 cells. 2023. SEAP reporter 293 cells expressing the human TLR2 and TLR1 genes. Catalog code: hkb-htlr21 [accessed May 16 2023]. https://www.invivogen.com/sites/default/files/invivogen/products/files/hek-blue_htlr2-tlr1_tds.pdf.

4. HKEB. 2023. Heat Killed E.coli 0111:B4 – TLR2 & TLR4 agonist. Catalog # tlrl-hkeb2 [accessed May 16 2023]. https://www.invivogen.com/sites/default/files/invivogen/products/files/hkeb_tds.pdf.

5. Utility of fatty acid profile and in vitro immune cell activation for chemical and biological standardization of Arthrospira/Limnospira

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