Molecular detection and phylogenetic analysis of a Shiga toxin-producing strain Escherichia coli (partial rfbE and fliCh7 gene), serotype O157:H7 isolated from a living chicken of a traditional market in Indonesia-Reference-Cited by-同舟云学术

Molecular detection and phylogenetic analysis of a Shiga toxin-producing strain Escherichia coli (partial rfbE and fliCh7 gene), serotype O157:H7 isolated from a living chicken of a traditional market in Indonesia

Published:2022 Issue:3 Volume:25 Page:500-506
ISSN:1313-3543
Container-title:BULGARIAN JOURNAL OF VETERINARY MEDICINE
language:
Short-container-title:BJVM

Author:

Ningrum S. G.¹,Khaerunnisa I.²,Supriyono ³,Wibawan I. W. T.⁴

Affiliation:

1. Faculty of Veterinary Medicine, Universitas Wijaya Kusuma Surabaya, Surabaya, Indonesia

2. Faculty of Agriculture, Bandung Raya University, Bandung, Indonesia

3. Laboratory of Medical Entomology, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia

4. Department of Animal Disease and Veterinary Public Health, IPB University, Bogor, Indonesia

Abstract

The objective of this study was to identify a Shiga toxin-producing strain Escherichia coli partial rfbE and fliCh7 gene of O157:H7 isolated from a faeces sample collected from a live chicken in a traditional market in Bogor, Indonesia. The isolate was investigated using multiplex polymerase chain reaction (PCR) to detect stx1, stx2, rfbE, and fliCh7 gene of STEC O157:H7. Then, sequencing was applied to identify the antigen markers, which are the rfbE and fliCh7 genes. In the present study, the isolate which was obtained from a live chicken was successfully identified as STEC O157:H7 strain. This conclusion was based on multiplex PCR and a nucleotide sequence analysis. This pathogen was not only found in the live chicken, but it was further suggested that the rfbE and fliCh7 genes can be used as alternative targets for molecular identification of this pathogen.

Publisher

Trakia University

Subject

General Veterinary

Reference22 articles.

1. Basli, A. A. A., M. M. Mohamadein & R. M. Farrag, 2016. Detection and virulence of Escherichia coli O157: H7 isolates using multiplex PCR. Wulfenia Journal, 23, 4148.

2. Beutin, L., D. Geier, H. Steinruck, S. Zimmermann & F. Scheutz, 1993. Prevalence and some properties of verotoxin (Shigalike toxin)-producing Escherichia coli in seven different species of healthy domestic animals. Journal of Clinical Microbiology, 31, 2483–2488.

3. Chinen, I., S. Epszteyn, C. L. Melamed, L. Aguerre, E. M. Espinosa, M. M Motter & M. Rivas, 2009. Shiga toxin-producing Escherichia coli O157 in beef and chicken burgers, and chicken carcasses in Buenos Aires, Argentina. International Journal of Food Microbiology, 132, 167171.

4. Darriba, D., G. L. Taboada, R. Doallo & D. Posada, 2012. jModelTest 2: More models, new heuristics and parallel computing. Nature Methods, 9, 772.

5. De Rauw, K., S. Jacobs & D. Pierard, 2018. Twenty-seven years of screening for Shiga toxin-producing Escherichia coli in a university hospital. Brussels, Belgium, 19872014. PloS One, 13(7).