Function and mechanism of action of the small regulatory RNA ArcZ inEnterobacterales

Abstract

ArcZ is a small regulatory RNA conserved inEnterobacterales. It is an Hfq-dependent RNA that is cleaved by RNase E in a processed form of 55–60 nucleotides. This processed form is highly conserved for controlling the expression of target mRNAs. ArcZ expression is induced by abundant oxygen levels and reaches its peak during the stationary growth phase. This control is mediated by the oxygen-responsive two-component system ArcAB, leading to the repression ofarcZtranscription under low-oxygen conditions in most bacteria in which it has been studied. ArcZ displays multiple targets, and it can control up to 10% of a genome and interact directly with more than 300 mRNAs inEscherichia coliandSalmonella enterica. ArcZ displays a multifaceted ability to regulate its targets through diverse mechanisms such as RNase recruitment, modulation of ribosome accessibility on the mRNA, and interaction with translational enhancing regions. By influencing stress response, motility, and virulence through the regulation of master regulators such as FlhDC or RpoS, ArcZ emerges as a major orchestrator of cell physiology withinEnterobacterales.