New high-throughput screening method for Chinese hamster ovary cell lines expressing low reduced monoclonal antibody levels: application of a system controlling the gas phase over cell lysates in miniature bioreactors and facilitating multiple sample setup
Author:
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Clinical Biochemistry,Biomedical Engineering,Bioengineering,Biotechnology
Link
https://link.springer.com/content/pdf/10.1007/s10616-023-00587-x.pdf
Reference32 articles.
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2. Chung WK, Russell B, Yang Y, Handlogten M, Hudak S, Cao M, Wang J, Robbins D, Ahuja S, Zhu M (2017) Effects of antibody disulfide bond reduction on purification process performance and final drug substance stability. Biotechnol Bioeng 114:1264–1274. https://doi.org/10.1002/bit.26265
3. Cura AJ, Xu X, Egan S, Aron K, Jenkins L, Hageman T, Huang Y, Chollangi S, Borys M, Ghose S, Li ZJ (2020) Metabolic understanding of disulfide reduction during monoclonal antibody production. Appl Microbiol Biotechnol 104:9655–9669. https://doi.org/10.1007/s00253-020-10916-1
4. Derfus GE, Dizon-Maspat J, Broddrick JT, Velayo AC, Toschi JD, Santuray RT, Hsu SK, Winter CM, Krishnan R, Amanullah A (2014) Red colored IgG4 caused by vitamin B12 from cell culture media combined with disulfide reduction at harvest. mAbs 6:679–688. https://doi.org/10.4161/mabs.28257
5. Du C, Huang Y, Borwankar A, Tan Z, Cura A, Yee JC, Singh N, Ludwig R, Borys M, Ghose S, Mussa N, Li ZJ (2018) Using hydrogen peroxide to prevent antibody disulfide bond reduction during manufacturing process. mAbs 10:500–510. https://doi.org/10.1080/19420862.2018.1424609
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