Abstract
Abstract
Background and aims
The pasture legume Trifolium subterraneum ssp. yanninicum exhibits waterlogging tolerance. This study investigates diversity for waterlogging tolerance within ssp. yanninicum. We tested the hypotheses that (1) variation for waterlogging tolerance exists within ssp. yanninicum and (2) is related to phenotypic and growth trait differences, which (3) reflect eco-geographic variables at site of origin.
Methods
Twenty-eight diverse ssp. yanninicum ecotypes collected from the Mediterranean region and four cultivars were grown in a controlled environment glasshouse. Seedling traits were measured at 14 and 21 days after sowing. Waterlogged and free-draining (control) treatments were then imposed for 28 days. Relative distance and multivariate plasticity indices were calculated.
Results
Under waterlogging, shoot (87–108% of controls) and root (80–116% of controls) relative growth rates (RGRs) differed significantly among ssp. yanninicum. Waterlogging tolerance, as assessed by shoot RGR, had strong positive correlations with root RGR (r = 0.86; P < 0.001), petiole length (r = 0.59; P < 0.001) and leaf size (r = 0.55; P < 0.01) under waterlogging. The proportion of biomass as leaf increased under waterlogging, due to leaf size being maintained (mean 102% of controls), but petiole length decreased (mean 84% of controls). Petiole length was the most plastic trait. Seed size, seedling traits, maturity duration and eco-geographic variables at site of origin were not related to waterlogging tolerance.
Conclusions
Wide variation in waterlogging tolerance exists within ssp. yanninicum. Petiole length reduction, an easy-to-measure and non-destructive indicator, could be used as a preliminary selection tool when screening large numbers of ssp. yanninicum for waterlogging tolerance in a breeding program.
Funder
University of Western Australia
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Soil Science
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