Abstract
AbstractRadiation of ionizing or non-ionizing nature has harmful effects on cellular components like DNA as radiation can compromise its proper integrity. To cope with damages caused by external stimuli including radiation, within living cells, several fast and efficient repair mechanisms have evolved. Previous studies addressing organismic radiation tolerance have shown that radiotolerance is a predominant property among extremophilic microorganisms including (hyper-) thermophilic archaea. The analysis of the ionizing radiation tolerance of the chemolithoautotrophic, obligate anaerobic, hyperthermophilic Crenarchaeon Ignicoccus hospitalis showed a D10-value of 4.7 kGy, fourfold exceeding the doses previously determined for other extremophilic archaea. The genome integrity of I. hospitalis after γ-ray exposure in relation to its survival was visualized by RAPD and qPCR. Furthermore, the discrimination between reproduction, and ongoing metabolic activity was possible for the first time indicating that a potential viable but non-culturable (VBNC) state may also account for I. hospitalis.
Funder
Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR)
Publisher
Springer Science and Business Media LLC
Subject
Genetics,Molecular Biology,General Medicine,Biochemistry,Microbiology
Reference52 articles.
1. Atienzar FA, Venier P, Jha AN, Depledge MH (2002) Evaluation of the random amplified polymorphic DNA (RAPD) assay for the detection of DNA damage and mutations. Mutat Res 521:151–163
2. Ayala-Torres S, Chen Y, Svoboda T, Rosenblatt J, Van Houten B (2000) Analysis of gene-specific DNA damage and repair using quantitative polymerase chain reaction. Methods 22:134–147
3. Baumstark-Khan C, Facius R (2001) Life under conditions of ionizing radiation. In: Horneck G, Baumstark-Khan C (eds) Astrobiology: the quest for the conditions of life. Springer, Berlin, pp 260–283
4. Beblo K, Rabbow E, Rachel R, Huber H, Rettberg P (2009) Tolerance of thermophilic and hyperthermophilic microorganisms to desiccation. Extremophiles 13:521–531
5. Beblo K, Douki T, Schmalz G, Rachel R, Wirth R, Huber H (2011) Survival of thermophilic and hyperthermophilic microorganisms after exposure to UV-C, ionizing radiation and desiccation. Arch Microbiol 193:797–809
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献