Metabolic integrity and stability of isolated rat brown adipocytes-Reference-Cited by-同舟云学术

Metabolic integrity and stability of isolated rat brown adipocytes

Published:1987-08-01 Issue:8 Volume:7 Page:659-666
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Van Voorhis Halina¹,Villa John J.¹,Otto David A.¹²

Affiliation:

1. Graduate Program in Nutrition, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08903

2. Department of Medical Education, Division of Research, The Baptist Medical Centers, 701 Princeton Avenue, Birmingham, Alabama 35211

Abstract

Using rats previously anesthetized with pentobarbital and with subtle modifications to existing procedures, rat brown adipocytes of good yield, viability, and stability were consistently obtained. The metabolic integrity of the cells, indicated by an 8–11 fold norepinephrine-dependent stimulation of respiration, was reproducible between cell preparations and maintained even after 3–5 hours of cell storage at room temperature. The ATP content of freshly isolated and stored cells per mg DNA (using a modified DNA assay) closely reflected the in vivo content determined from freeze clamped tissue.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/7/8/659/472084/bsr0070659.pdf

Reference21 articles.

1. Bukowiecki, L., Collet, A. J., Follea, N., Guay, G. and Jahjah, L. (1982).Am. J. Physiol. 242:E353-E359.

2. Bukowiecki, L. J. and Collet, A. J. (1983).J. Obesity and Weight Regulation 2:29?53.

3. Bukowiecki, L., Follea, N., Paradis, A. and Collet, A. (1980).Am. J. Physiol. 238:E552-E563.

4. Burton, K. (1956).Biochem. J. 62:315?323.

5. Cooney, G. J. and Newsholme, E. A. (1984).TIBS 9:303?305.

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. L-alanine transport in isolated cells of interscapular brown adipose tissue in rat;Bioscience Reports;1991-04-01