Amniotic Fluid Proteomics Analysis and In Vitro Validation to Identify Potential Biomarkers of Preterm Birth
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Published:2024-03-07
Issue:7
Volume:31
Page:2032-2042
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ISSN:1933-7191
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Container-title:Reproductive Sciences
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language:en
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Short-container-title:Reprod. Sci.
Author:
Chen SiguoORCID, Wang Weizhou, Yan Guanghong, Liu Mengmei, Li Min, Chen Ping, Ma Qingyan, Zhang Jinman, Tang Ying, Zhou Linglin, You Dingyun
Abstract
AbstractThis study aimed to investigate the regulation of amniotic fibroblast (AFC) function by vitamin K–dependent protein Z (PROZ) during preterm birth (PTB) and its potential role in adverse pregnancy outcomes. Proteomic samples were collected from amniotic fluid in the second trimester, and AFC were isolated from the amniotic membrane and cultured in vitro. The expression of extracellular and intracellular PROZ in AFC was modulated, and their biological properties and functions were evaluated. Clinical analysis revealed a significant upregulation of PROZ expression in amniotic fluid from preterm pregnant women. In vitro experiments demonstrated that PROZ stimulated AFC migration, enhanced their proliferative capacity, and reduced collagen secretion. Overexpression of PROZ further enhanced cell migration and proliferation, while knockdown of PROZ had the opposite effect. PROZ plays a crucial role in promoting the proliferation and migration of amniotic membrane fibroblasts. Increased PROZ expression in amniotic fluid is associated with the occurrence of PTB. These findings shed light on the potential involvement of PROZ in adverse pregnancy outcomes and provide a basis for further research on its regulatory mechanisms during PTB.
Funder
National Natural Science Foundation of China Innovative Research Group Project of the National Natural Science Foundation of China Basic Research of Yunnan Province - Key Project Applied Basic Research Foundation of Yunnan Province Special Project for Selection of High-level Scientific and Technological Talents and Innovation Team-Reserve Talent Program for Young and Middle-aged Academic and Technical Leaders Basic Research of Yunnan Province Joint Special Project of Kunming Medical University
Publisher
Springer Science and Business Media LLC
Reference39 articles.
1. Medley N, Vogel JP, Care A, Alfirevic Z. Interventions during pregnancy to prevent preterm birth: an overview of Cochrane systematic reviews. Cochrane Database Syst Rev. 2018;11(11):CD012505. 2. Goldenberg RL, Culhane JF, Iams JD, Romero R. Epidemiology and causes of preterm birth. Lancet. 2008;371(9606):75–84. 3. Koullali B, van Zijl MD, Kazemier BM, Oudijk MA, Mol BWJ, Pajkrt E, Ravelli ACJ. The association between parity and spontaneous preterm birth: a population based study. BMC Pregnancy Childbirth. 2020;20(1):233. 4. Brown RG, Marchesi JR, Lee YS, Smith A, Lehne B, Kindinger LM, Terzidou V, Holmes E, Nicholson JK, Bennett PR, MacIntyre DA. Vaginal dysbiosis increases risk of preterm fetal membrane rupture, neonatal sepsis and is exacerbated by erythromycin. BMC Med. 2018;16(1):9. 5. Zhuang L, Li ZK, Zhu YF, Ju R, Hua SD, Yu CZ, Li X, Zhang YP, Li L, Yu Y, Zeng W, Cui J, Chen XY, Peng JY, Li T, Feng ZC. The correlation between prelabor rupture of the membranes and neonatal infectious diseases, and the evaluation of guideline implementation in China: a multicenter prospective cohort study. Lancet Reg Health West Pac. 2020;17(3):100029.
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