Abstract
AbstractBlackleg disease, caused by Leptosphaeria maculans, greatly affects the production of cabbage (Brassica oleracea). However, definitive R-gene(s) are yet to be identified in this crop. In contrast, a number of R-loci have been identified in A- or B-genome crops. Identification of few resistant cabbage genotypes indicates the presence of R-genes in this C-genome crop. High ancestral synteny between Brassica genomes suggests that the collinear regions of known A- or B-genome R-loci may also contain functional R-genes in the C-genome. Strong resistance was observed in the cotyledons of cabbage inbred line SCNU-98 against two L. maculans isolates, 03–02 s and 00–100 s. We investigated the collinear region of the Brassica napus blackleg resistance locus LepR2’ in B. oleracea since both isolates of L. maculans contain corresponding avirulence genes. The locus was collinear to a 5.8 Mbp genomic segment of B. oleracea chromosome C09 containing 13 genes that have putative disease resistance-related domains. High expression of genes Bo9g117290 and Bo9g111510 against isolate 00–100 s, and high expression of genes Bo9g126150 and Bo9g111490 against both isolates in the resistant-line SCNU-98 indicate their putative roles in blackleg resistance, which remained to be functionally verified. This work enhances our understanding of R-gene-mediated resistance to blackleg in cabbage.
Funder
Ministry of Agriculture, Food and Rural Affairs
Publisher
Springer Science and Business Media LLC
Subject
Horticulture,Plant Science,Biotechnology
Reference72 articles.
1. Alamery S, Tirnaz S, Bayer P, Tollenaere R, Chaloub B, Edwards D, Batley J (2018) Genome-wide identification and comparative analysis of NBS-LRR resistance genes in Brassica napus. Crop Pasture Sci 69:79–93
2. Ananga AO, Cebert E, Soliman K, Kantety R, Pacumbaba RP, Konan K (2006) RAPD markers associated with resistance to blackleg disease in Brassica species. J Biotechnol 5:2041–2048
3. Ansan-Melayah D, Balesdent MH, Delourme R, Pilet ML, Tanguy X, Renard M, Rouxel T (1998) Genes for race-specific resistance against blackleg disease in Brassica napus L. Plant Breed 117:373–378
4. Badawy HMA, Hoppe H, Koch E (1991) Differential reactions between the genus Brassica and aggressive single spore isolates of Leptosphaeria maculans. J Phytopathol 131:109–119
5. Balesdent MH, Attard A, Kühn ML, Rouxel T (2002) New avirulence genes in the phytopathogenic fungus Leptosphaeria maculans. Genet Resist 92:1122–1133
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