Pitfalls in the normalization of real-time polymerase chain reaction data-Reference-Cited by-同舟云学术

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Pitfalls in the normalization of real-time polymerase chain reaction data

Published:2007-03-19 Issue:3 Volume:102 Page:
ISSN:0300-8428
Container-title:Basic Research in Cardiology
language:en
Short-container-title:Basic Res Cardiol

Author:

Hendriks-Balk M. C.,Michel M. C.,Alewijnse A. E.

Publisher

Springer Science and Business Media LLC

Subject

Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology

Link

https://link.springer.com/content/pdf/10.1007/s00395-007-0649-0.pdf

Reference10 articles.

1. Brattelid T, Tveit K, Birkeland JAK, Sjaastad I, Qvigstad E, Krobert KA, Hussain RI, Skomedal T, Osnes J, Levy FO (2007) Expression of mRNA encoding G protein- coupled receptors involved in congestive heart failure: a quantitative RTPCR study and the question of normalisation. Basic Res Cardiol (DOI 10.1007/s00395-007-0648-1)

2. Bustin SA, Nolan T (2004) Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J Biomol Tech 15:155–166

3. Dheda K, Huggett J, Chang JS, Kim LU, Bustin SA, Johnson MA, Rook GAW, Zumla A (2005) The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal Biochem 344:141–143

4. Huggett J, Dheda K, Bustin SA, Zumla A (2005) Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 6:279–284

5. de Kok JB, Roelofs RW, Giesendorf BA, Pennings JL, Waas ET, Feuth T, Swinkels DW, Span PN (2005) Normalization of gene expression measurements in tumour tissues: comparison of 13 endogenous control genes. Lab Invest 85:154–159

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