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Characterization of the complete genome of euonymus yellow vein associated virus, a distinct member of the genus Potexvirus, family Alphaflexiviridae, isolated from Euonymus bungeanus Maxim in Liaoning, Northern China

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Abstract

In August 2016, a yellow vein disease was observed on leaves of Euonymus bungeanus Maxim (Euonymus, Celastraceae) in Liaoning, China. Virions measuring 750 × 13 nm were observed in a sample from the diseased plant. A potexvirus was detected in the sample by small-RNA deep sequencing analysis and recovered by traditional cloning. The genome of this potexvirus consists of 7,279 nucleotides, excluding the poly(A) tail at the 3’ end, and contains five open reading frames (ORFs). Based on the nucleotide and amino acid sequences of the coat protein gene, the virus shared the highest sequence similarity with white clover mosaic virus (WCMV, X16636) (40.1%) and clover yellow mosaic virus (ClYMV, D00485) (37.1%). Phylogenetic analysis showed that the virus clustered with potexviruses and is most closely related to strawberry mild yellow edge virus. These results indicate that this virus is a distinct member of the genus Potexvirus, for which the name euonymus yellow vein associated virus (EuYVAV) is proposed. To our knowledge, this is the first report of a potexvirus on E. bungeanus.

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Acknowledgements

This work was supported by funds from the Natural Science Foundation of Liaoning Province (2015020806) and the General Project of Liaoning Provincial Department of Education (L2015362). We thank Zhenguo Du and Fangluan Gao, Institute of Plant Virology and the Fujian Agriculture and Forestry University, for useful discussions and help in constructing the phylogenetic tree.

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Authors and Affiliations

  1. Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Engineering, Shenyang University, Dadong, Shenyang, 110044, Liaoning, China

    Caixia Yang, Tong Han, Jingjing Fu & Yiming Liao

  2. School of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou, 412007, Hunan, China

    Sha Chen

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  1. Caixia Yang
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  2. Tong Han
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  3. Jingjing Fu
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  4. Yiming Liao
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  5. Sha Chen
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Correspondence to Caixia Yang or Sha Chen.

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Handling Editor: F. Murilo Zerbini.

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Yang, C., Han, T., Fu, J. et al. Characterization of the complete genome of euonymus yellow vein associated virus, a distinct member of the genus Potexvirus, family Alphaflexiviridae, isolated from Euonymus bungeanus Maxim in Liaoning, Northern China. Arch Virol 163, 563–566 (2018). https://doi.org/10.1007/s00705-017-3617-5

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  • DOI: https://doi.org/10.1007/s00705-017-3617-5

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