Abstract
AbstractDoubled haploidy is the fastest way of achieving completely homozygous plants in one generation. It shortens the time required for the development of mutant varieties allowing rapid response against threats to sustainable food production caused by changing climate and stresses due to emerging pests like Striga. In this chapter, we describe step-by-step protocols to obtain doubled haploid rice with fixed novel mutant variants by anther culture from gamma ray irradiated M1 populations to accelerate mutation breeding for resistance to Striga in rice.
Publisher
Springer Berlin Heidelberg
Reference9 articles.
1. Afza R, Shen M, Zapata-Arias FJ, Xie J, Fundi HK, Lee K-S, Bobadilla-Mucino E, Kodym A (2000) Effect of spiklet position on rice anther culture efficiency. Plant Sci 153:155–159
2. Brunner H (1995) Radiation induced mutations for plant selection. Appl Radiat Isot 46:589–594
3. Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Pi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sinica 18:659–668
4. Germana MA (2011) Anther culture for haploid and doubled haploid production. Plant Cell, Tissue Organ Cult 104:283–300
5. Khound R, Santra M, Baenziger PS, Santra DK (2013) Effect of cold-mediated pretreatment on microspore culture in winter and spring wheat. Am J Plant Sci 4:2259–2264