Proteomic investigation of neural stem cell to oligodendrocyte precursor cell differentiation reveals phosphorylation-dependent Dclk1 processing
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Published:2023-08-18
Issue:9
Volume:80
Page:
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ISSN:1420-682X
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Container-title:Cellular and Molecular Life Sciences
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language:en
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Short-container-title:Cell. Mol. Life Sci.
Author:
Hardt RobertORCID, Dehghani AlirezaORCID, Schoor Carmen, Gödderz MarkusORCID, Cengiz Winter NurORCID, Ahmadi Shiva, Sharma Ramesh, Schork KarinORCID, Eisenacher MartinORCID, Gieselmann VolkmarORCID, Winter DominicORCID
Abstract
AbstractOligodendrocytes are generated via a two-step mechanism from pluripotent neural stem cells (NSCs): after differentiation of NSCs to oligodendrocyte precursor/NG2 cells (OPCs), they further develop into mature oligodendrocytes. The first step of this differentiation process is only incompletely understood. In this study, we utilized the neurosphere assay to investigate NSC to OPC differentiation in a time course-dependent manner by mass spectrometry-based (phospho-) proteomics. We identify doublecortin-like kinase 1 (Dclk1) as one of the most prominently regulated proteins in both datasets, and show that it undergoes a gradual transition between its short/long isoform during NSC to OPC differentiation. This is regulated by phosphorylation of its SP-rich region, resulting in inhibition of proteolytic Dclk1 long cleavage, and therefore Dclk1 short generation. Through interactome analyses of different Dclk1 isoforms by proximity biotinylation, we characterize their individual putative interaction partners and substrates. All data are available via ProteomeXchange with identifier PXD040652.
Funder
Deutsche Forschungsgemeinschaft Deutscher Akademischer Austauschdienst Medizinische Fakultät, Rheinische Friedrich-Wilhelms-Universität Bonn Bundesministerium für Bildung und Forschung CUBiMed.RUB Rheinische Friedrich-Wilhelms-Universität Bonn
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Cellular and Molecular Neuroscience,Pharmacology,Molecular Biology,Molecular Medicine
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