Author:
Zhang Lihua,Guo Hui,Zhang Xiaoguang,Wang Ling,Wei Feng,Zhao Yike,Wang Bo,Meng Yibo,Li Yanling
Abstract
AbstractChronic psoriasis is a kind of immune-mediated skin illness and the underlying molecular mechanisms of pathogenesis remain incompletely understood. Here, we used small RNA microarray assays to scan the differential expressed RNAs in psoriasis patient samples. The downstream miRNAs and its targets were predicted using bioinformatics analysis from online bases and confirmed using fluorescence in situ hybridization and dual‑luciferase report gene assay. Cell ability of proliferation and migration were detected using CCK-8 and transwell assays. The results showed that a new snoRNA Snora73 was upregulated in psoriasis patient samples. Overexpression of Snora73 significantly increased psoriasis cells viability and migration, while knockdown of Snora73 got the opposite results. Mechanistically, our results showed that Snora73 acted as a sponge for miR-3074-5p and PBX1 is a direct target of miR-3074-5p in psoriasis cells. Furthermore, miR-3074-5p suppressed psoriasis cell proliferation and migration, while PBX1 promoted cell proliferation and migration in psoriasis. Collectively, these findings reveal a crucial role of Snora73 in progression of psoriasis through miR-3074-5p/PBX1 signaling pathway and suggest a potential therapeutic strategy.
Funder
Hebei Provincial Clinical Medical Research Center for Dermatology and Venereal Diseases Innovation Capacity Enhancement
Epidemiological characteristics of psoriasis and prediction and prevention of psoriasis co-morbidities in Hebei Province
The Health and Family Planning Commission of Hebei Province
Hebei Provincial Pharmaceutical Society Hospital Pharmacy Special Research Project
Publisher
Springer Science and Business Media LLC
Subject
Genetics,General Medicine