Abstract
AbstractPlant responses to phosphate (Pi) starvation stress involve an array of adaptive strategies including enhanced accumulation and shoot-to-root transport of carbohydrates required for activating the plant Pi starvation signaling. However, the contribution of carbohydrate biosynthesis genes to maintaining phosphorus (P) homeostasis remains unknown, and the functional characterization of sugar metabolism genes is often impeded or compromised due to the loss of fertility of the null mutants. Here, a highly expressed gene encoding UDP-glucose pyrophosphorylase in rice (Oryza sativa), Ugp1, was functionally characterized. Ugp1 was transcriptionally induced in leaf blades by Pi starvation. As a link between sucrose (Suc) and P, we assumed that overexpression of Upg1 would alter the Suc concentration. Given that Suc is a signaling molecule, such concentration changes would affect the P deficiency signal transduction, thereby altering the homeostasis of endogenous P. The results showed that, overexpression of Ugp1 decreased plant biomass, increased sucrose content, and promoted Pi accumulation. The elevated Pi accumulation in Ugp1-OX plants was accompanied by the up-regulation of Pi-starvation-induced genes. Taken together, these results demonstrate that Ugp1 is a positive regulator of sucrose accumulation, and is required for maintaining P homeostasis in rice. Ugp1 thus represents a novel entry point to dissect the mechanisms underlying the carbon-P crosstalk.
Funder
Natural Science Research of Jiangsu Higher Education Institutions of China
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Molecular Biology,Physiology
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献