Abstract
AbstractDiabetes mellitus (DM) is a chronic and progressive metabolic disorder that can stimulate neuroinflammation and increase oxidative stress in the brain. Therefore, the present study was aimed to assess the efficacy of ethanolic Terminalia chebula extract against the neurochemical and histopathological changes induced in the brains of diabetic rats. The study clarified the reduction in oxidative stress induced in the brains of diabetic rats by the significant (P ≤ 0.05) increase in levels of the antioxidants with decreasing the peroxidation products via ethanolic T. chebula extract at both doses (400 and 600 mg/kg). Moreover, T. chebula extract improved the brain integrity by lowering levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), β-amyloid (Aβ) content, monocyte chemoattractant protein-1 (MCP-1) and acetylcholine esterase (ACHE) significantly (P ≤ 0.05) in a dose dependent manner compared to brain of diabetic rats. Severe nuclear pyknosis and degeneration were noticed in neurons of the cerebral cortex, hippocampus and striatum in brains of diabetic rats. The severity of these alterations decreased with T. chebula extract at a dose of 600 mg/kg compared to the other treated groups. The different electrophoretic protein and isoenzyme assays revealed that the lowest similarity index (SI%) values exist in the brains of diabetic rats compared to the control group. The quantity of the most native proteins and isoenzyme types increased significantly (P ≤ 0.05) in the brains of diabetic rats, and these electrophoretic variations were completely diminished by T. chebula extract. The study concluded that T. chebula extract ameliorated the biochemical, histopathological and electrophoretic abnormalities induced in the brains of diabetic rats when administered at a dose of 600 mg/kg.
Funder
National Research Centre Egypt
Publisher
Springer Science and Business Media LLC
Reference92 articles.
1. Abd Elhalim SA, Sharada HM, Abulyazid I, Aboulthana WM, Abd Elhalim ST (2017) Ameliorative effect of carob pods extract (Ceratonia siliqua L.) against cyclophosphamide induced alterations in bone marrow and spleen of rats. J Appl Pharm Sci 7(10):168–181
2. Abdel-Halim AH, Fyiad AA, Aboulthana WM, El-Sammad NM, Youssef AM, Ali MM (2020) Assessment of the anti-diabetic effect of Bauhinia variegata gold nano-extract against streptozotocin induced diabetes mellitus in rats. J Appl Pharm Sci 10(05):077–091
3. Abdel-Salam OME, Nada SO, Salem N, El-Shamarka ME, Omara E (2014) Effect of Cannabis sativa on oxidative stress and organ damage after systemic endotoxin administration, in mice com. Comp Clin Path 23:1069–1085
4. Aboulthana WM, Ismael M, Farghaly HS (2016) Assessment of mutagenicity induced by toxic factors affecting ovarian tissue in rats by electrophoresis and molecular dynamic modeling. Int J Curr Pharm Rev Res 7(6):347–359
5. Aboulthana WM, El-Feky AM, Ibrahim NE, Sahu RK, El-Sayed AB (2018) Evaluation of the pancreatoprotective effect of Nannochloropsis oculata extract against streptozotocin-induced diabetes in rats. J Appl Pharm Sci 8(06):046–058
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献