Application of recyclable CRISPR/Cas9 tools for targeted genome editing in the postharvest pathogenic fungi Penicillium digitatum and Penicillium expansum

Author:

Garrigues SandraORCID,Manzanares Paloma,Marcos Jose F.

Funder

Conselleria d'Educació, Investigació, Cultura i Esport

Ministerio de Ciencia e Innovación

Publisher

Springer Science and Business Media LLC

Subject

Genetics,General Medicine

Reference74 articles.

1. Al Abdallah Q, Ge W, Fortwendel-Jarrod R, Mitchell-Aaron P (2017) A simple and universal system for gene manipulation in Aspergillus fumigatus: in vitro-assembled Cas9-guide RNA ribonucleoproteins coupled with microhomology repair templates. mSphere 2:e00446-00417. https://doi.org/10.1128/mSphere.00446-17

2. Allen F, Crepaldi L, Alsinet C, Strong AJ, Kleshchevnikov V, De Angeli P, Páleníková P, Khodak A, Kiselev V, Kosicki M, Bassett AR, Harding H, Galanty Y, Muñoz-Martínez F, Metzakopian E, Jackson SP, Parts L (2019) Predicting the mutations generated by repair of Cas9-induced double-strand breaks. Nat Biotechnol 37:64–72. https://doi.org/10.1038/nbt.4317

3. Arazoe T, Miyoshi K, Yamato T, Ogawa T, Ohsato S, Arie T, Kuwata S (2015) Tailor-made CRISPR/Cas system for highly efficient targeted gene replacement in the rice blast fungus. Biotechnol Bioeng 112:2543–2549. https://doi.org/10.1002/bit.25662

4. Ballester A-R, Marcet-Houben M, Levin E, Sela N, Selma-Lázaro C, Carmona L, Wisniewski M, Droby S, González-Candelas L, Gabaldón T (2014) Genome, transcriptome, and functional analyses of Penicillium expansum provide new insights into secondary metabolism and pathogenicity. Mol Plant-Microbe Interact 28:232–248. https://doi.org/10.1094/mpmi-09-14-0261-fi

5. Ballester A-R, López-Pérez M, de la Fuente B, González-Candelas L (2019) Functional and pharmacological analyses of the role of Penicillium digitatum proteases on virulence. Microorganisms 7:7. https://doi.org/10.3390/microorganisms7070198

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