Abstract
AbstractUnderstanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (ALFA), and a corresponding nanobody fused to mEos3.2. The methodology presented is applicable to other bacterial proteins.
Funder
Okinawa Institute of Science and Technology Graduate University
Novo Nordisk Fonden
Carl Tryggers Stiftelse för Vetenskaplig Forskning
Australian Research Council
Japan Society for the Promotion of Science
University of Technology Sydney
Publisher
Springer Science and Business Media LLC
Subject
Genetics,General Medicine
Cited by
3 articles.
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