Optimized RNA Extraction and Northern Hybridization in Streptomycetes
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Biochemistry, Genetics and Molecular Biology
Link
http://www.springerlink.com/index/pdf/10.1007/s12575-010-9027-7
Reference9 articles.
1. Bucca G, Ferina G, Puglia AM, Smith CP (1995) The dnaK operon of Streptomyces coelicolor encodes a novel heat-shock protein which binds to the promoter region of the operon. Mol Microbiol 17:663–674
2. Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA (2000) Practical Streptomyces genetics. John Innes Foundation, Norwich
3. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159
4. Burnett WV (1997) Northern blotting of RNA denatured in glyoxal without buffer recirculation. Biotechniques 22:668–671
5. Chomczynski P (1992) One-hour downward alkaline capillary transfer for blotting of DNA and RNA. Anal Biochem 201:134–139
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