Abstract
Abstract
When considering mold prevention strategies, the environmental conditions in which fungi grow need to be taken into consideration. This environment is often characterized by a time-dependent relative humidity, and porous substrate. Growth has mainly been investigated in steady-state experiments. Therefore, the goal of this study is to understand the hyphal growth of Penicillium rubens on porous gypsum, under dynamic humidity conditions. Spores of P. rubens were inoculated on porous gypsum containing nutrients, and placed in a small incubation chamber, allowing for microscopic hyphal observation. The relative humidity in this chamber varied multiple times between a high (close to 100%) and low value (35%, 55%, or 75%). The hyphae reacted to a lowered relative humidity by an immediate growth stop and dehydration. When the relative humidity was increased again, the hyphae re-hydrated and three responses were found: regrowing after approximately 4 h, after a time equal to the germination time, or no regrowth at all. No substantial regrowth was found for fluctuations faster than 4 h. This time-scale was found for multiple decreases in relative humidity, and has been reported for the first time.
Key points
• Hyphae restart growth after a characteristic time of approximately 4 h.
• Relative humidity fluctuations of 3 h can suppress hyphal growth.
• Hyphae do not regrow after a severe desiccation and short periods of high humidity.
Funder
Stichting voor de Technische Wetenschappen
Ministerie van Economische Zaken
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Reference50 articles.
1. Adan OCG (1994) On the fungal defacement of interior finishes, Dissertation. Technische Universiteit Eindhoven
2. Adan OCG, Huinink HP, Bekker M (2011) Water relations of fungi in indoor environments. Wageningen Academic Publishers, Wageningen
3. Andersen B, Frisvad JC, Søndergaard I, Rasmussen IS, Larsen LS (2011) Associations between fungal species and water-damaged building materials. Appl Environ Microbiol 77(12):4180–4188
4. Ayerst G (1969) The effects of moisture and temperature on growth and spore germination in some fungi. J Stored Prod Res 5(2):127–141
5. Bekker M (2014) Growth of Penicillium rubens after desiccation, Dissertation. Technische Universiteit Eindhoven
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献