Solubility enhancement of Cry2Aa crystal through carboxy-terminal extension and synergism between the chimeric protein and Cry1Ac
Author:
Publisher
Springer Science and Business Media LLC
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Link
http://link.springer.com/content/pdf/10.1007/s00253-018-09606-w.pdf
Reference34 articles.
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3. Bian X, Huang F, Stewart FA, Xia L, Zhang Y, Müller R (2012) Direct cloning, genetic engineering, and heterologous expression of the syringolin biosynthetic gene cluster in E. coli through red/ET recombineering. Chembiochem 13:1946–1952
4. Carmona AA, Ibarra JE (1999) Expression and crystallization of a Cry3Aa-Cry1Ac chimerical protein of Bacillus thuringiensis. World J Microbiol Biotechnol 15:455–463
5. Du C, Martin PA, Nickerson KW (1994) Comparison of disulfide contents and solubility at alkaline pH of insecticidal and noninsecticidal Bacillus thuringiensis protein crystals. Appl Environ Microbiol 60:3847–3853
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