Abstract
AbstractMultiplexed imaging, which enables spatial localization of proteins and RNA to cells within tissues, complements existing multi-omic technologies and has deepened our understanding of health and disease. CODEX, a multiplexed single-cell imaging technology, utilizes a microfluidics system that incorporates DNA barcoded antibodies to visualize 50 + cellular markers at the single-cell level. Here, we discuss the latest applications of CODEX to studies of cancer, autoimmunity, and infection as well as current bioinformatics approaches for analysis of multiplexed imaging data from preprocessing to cell segmentation and marker quantification to spatial analysis techniques. We conclude with a commentary on the challenges and future developments for multiplexed spatial profiling.
Funder
National Cancer Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases
Rheumatology Research Foundation
School of Medicine, Stanford University
Stanford Cancer Institute
Publisher
Springer Science and Business Media LLC
Subject
Immunology,Immunology and Allergy
Cited by
9 articles.
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