Using isothermal calorimetry and FT-Raman spectroscopy for step-by-step monitoring of maize seed germination: case study

Abstract

AbstractThermoanalytical methods are widely used to study seed germination processes. Our research aimed to use them in order to monitor the germination of maize seeds. To this end, we employed—for the first time—the isothermal calorimetric method with full oxygen access. Dry seeds were placed in ampoules with threaded caps, filled with distilled water. Heat flow was recorded in microwatts at 20 °C for 72 h in order to determine changes in thermal power associated with germination. In parallel with these measurements, in the same time regime, we measured FT-Raman spectra, which allowed us to analyze how endosperm and embryo changed their chemical compositions during imbibition and germination. Spectra obtained for endosperm reflected chemical changes resulting from catabolic processes. We also analyzed variations in the secondary structure of proteins in the embryo accompanying germination. Decomposition of amide I bands proved that during germination and protein hydrolysis, the content of helical and β-sheet structures decreased. Furthermore, in the embryos of dry seeds, S-S linkages were in both gauche–gauche-trans and trans-gauche-trans conformations, but after imbibition, only gauche–gauche-trans conformation can be identified.