Author:
Sgrò Salvatore,Lavezzi Benedicta,Caprari Cristian,Polito Marco,D’Elia Marcello,Lago Giampietro,Furlan Giada,Girotti Stefano,Ferri Elida Nora
Abstract
AbstractHemp cultivation is living a period of renewed interest worldwide after long years of opposition and abandonment. The European Union (EU) allows and subsidizes the growing of fiber and oilseed cultivars of Cannabis sativa L. with respect to the THC content limit of 0.2%. The EU method for the quantitative determination of Δ9-tetrahydrocannabinol (THC) content in hemp varieties provides to apply a tolerance of 0.03 g of THC per 100 g of sample concerning compliance assessment to that limit. However, the method does not report any precision data, especially useful as a function of THC content to evaluate measurement uncertainty and therefore to establish the conformity of hemp at different THC legal limits. Measurement uncertainty of the method by both bottom-up and top-down approach, besides repeatability and reproducibility, was investigated and estimated in the THC concentration range 0.2–1.0%, which includes the different legal limits set out for hemp around the world. We proposed Decision Rules for conformity of hemp showing that a non-compliant declaration beyond reasonable doubt should be stated when the THC content, as a mean result on a duplicate analysis, exceeds the limit by at least 11–15%, depending on THC limit. We highlighted other issues concerning practical aspects of hemp analysis, from sampling to evaluation of results, as well as the need to carry out collaborative studies on the EU method.
Graphical abstract
Funder
Alma Mater Studiorum - Università di Bologna
Publisher
Springer Science and Business Media LLC
Subject
Biochemistry,Analytical Chemistry
Reference57 articles.
1. Small E. Cannabis: a complete guide. Boca Raton: CRC Press; 2017.
2. Andre CM, Hausman JF, Guerriero G. Cannabis sativa: the plant of the thousand and one molecules. Front Plant Sci. 2016;7:19. https://doi.org/10.3389/fpls.2016.00019.
3. WHO. Report of the World Health Organization Alcohol, Drugs, and Addictive Behaviours Unit on Cannabis. https://www.who.int/substance_abuse/facts/cannabis/en. Accessed 15 Jan 2021.
4. Brighenti V, Protti M, Anceschi L, Zanardi C, Mercolini L, Pellati F. Emerging challenges in the extraction, analysis and bioanalysis of cannabidiol and related compounds. J Pharm Biomed Anal. 2020;192:113633. https://doi.org/10.1016/j.jpba.2020.113633.
5. Citti C, Ciccarella G, Braghiroli D, Parenti C, Vandelli MA, Cannazza G. Medicinal cannabis: principal cannabinoids concentration and their stability evaluated by a high performance liquid chromatography coupled to diode array and quadrupole time of flight mass spectrometry method. J Pharm Biomed Anal. 2016;128:201–9. https://doi.org/10.1016/j.jpba.2016.05.033.
Cited by
21 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献