Modified protocols for rapid carrot genomic DNA extraction and AFLP™ analysis using silver stain or radioisotopes

Author:

Briard M.,Le Clerc V.,Grzebelus D.,Senalik D.,Simon P. W.

Publisher

Springer Science and Business Media LLC

Subject

Plant Science,Molecular Biology

Reference6 articles.

1. Bassam BJ, Caetano-Anolles G and Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196: 80–83.

2. Boiteux LS, Fonseca MEN and Simon PW (1999) Effects of plant tissue, and DNA purification method on randomly amplified polymorphic DNA-based genetic fingerprinting analysis in carrot. J Amer Soc Hort Sci 124: 32–38.

3. Chalhoub BA, Thibault S, Laucou V, Rameau C, Hofte H and Cousin R (1997) Silver staining and recovery of AFLP amplification products on large denaturing polyacrylamide gels. Bio Techniques 22: 216–220.

4. Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual. 2nd/Ed. CSH Laboratory Press, Cold Spring Harbor, New York.

5. Vivek BS, and Simon PW (1999) Linkage analysis among molecular markers and storage root traits of carrot (Daucus carota L. ssp.sativus). Theor Appl Genet 99: 58–64.

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