RAPD analysis in flax: Optimization of yield and reproducibility using klenTaq 1 DNA polymerase, chelex 100, and gel purification of genomic DNA

Author:

Aldrich Jane,Cullis Christopher A.

Publisher

Springer Science and Business Media LLC

Subject

Plant Science,Molecular Biology

Reference12 articles.

1. Aldrich, J., and C.A. Cullis. 1992. Enhanced screening of RAPDS in flax lines nearly isogenic for the L resistance gene using low melting agarose for DNA purification and KlenTaq 1 for PCR. Plant Genome I, The International Conference on the Plant Genome, San Diego, CA. p. 16.

2. Anonymous. 1991. Stoffel fragment: Increased thermal stability and broad Mg2+ optimum. Amplifications: A Forum for PCR Users, Issue 6: 14.

3. Barnes, W.M. 1992. The fidelity ofTaq polymerase catalyzing PCR is improved by an N-terminal deletion. Gene 112:29–35.

4. Bult, C., M. Källersjo, and S. Youngbae. 1992. Amplification and sequencing of 16/18S rDNA from gel-purified total plant DNA. Plant Mol. Biol. Rep. 10:273–284.

5. Caetano-Anolles, G., B.J. Bassam, and P. M. Gresshoff. 1991. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Bio/Technology 9, 553–557.

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