Affiliation:
1. Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China
2. Department of Obstetrics and Gynecology, Panyu District Zhongcun Hospital, Guangzhou 511495, China
3. Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou 510632, China
Abstract
Abstract
Objective
We aimed to evaluate the expression pattern of the genes BIM, BCL-6, and c-MYC in adult patients at initial diagnosis of B-cell acute lymphoblastic leukemia (B-ALL).
Methods
Relative mRNA levels of BIM, BCL-6, and c-MYC in peripheral blood mononuclear cells (PBMCs) from B-ALL patients were determined by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) using SYBR Green dye. PBMCs from healthy volunteers served as a control. GAPDH was used as a reference gene.
Results
Relative expression of BIM, BCL-6, and c-MYC mRNA in B-ALL patients was significantly lower than in healthy controls (P < 0.05). Furthermore, this result was observed for both newly diagnosed B-ALL patients and those incomplete remission (CR) (P < 0.05). There were no statistically significant differences in the expression levels of BIM, BCL-6, and c-MYC between these B-ALL patient groups (P > 0.05). Spearman’s rank correlation analyses revealed the expression level of BIM to be positively correlated with that of BCL-6 in B-ALL patients.
Conclusion
Expression of the genes BIM, BCL-6, and c-MYC is decreased in adult B-ALL patients. Moreover, the expression pattern of these genes may be similar in such patients at initial diagnosis and following CR. The expression characteristics of BIM, BCL-6, and c-MYC may constitute useful markers for the diagnosis of adult B-ALL.
Publisher
Ovid Technologies (Wolters Kluwer Health)