Response surface methodology: Optimization of myco-synthesized gold and silver nanoparticles by Trichoderma saturnisporum

Abstract

AbstractIn the current study, the optimal reaction condition for fabrication of Au and Ag nanoparticles by using Trichoderma saturnisporum was developed. Optimization of fermentation conditions for extracellular AuNPs and AgNPs synthesis using response surface methodology was achieved. To accomplish the ideal reaction state, the green synthesis reaction’s effective parameters and central composite face design were used. Precursor concentrations, pH, and temperature were found to be the most effective parameters for the fabrication of Au and Ag nanomaterials. The model’s realization used a respectable foot. Normal probability plots; interaction plots, including effect plots; variance analysis (ANOVA), surface plots; contour plots; and Pareto charts were used to optimize the components. Significance threshold (5%) was used to influence all of the factors. The production process was also influenced by some of the potential connections between these factors. The recommended regression model fits the experimental data extremely well. The findings of this study looked at how much more bio-fabrication was possible with optimal fermentation conditions for the producer of AuNPs and AgNPs compared with traditional optimization techniques.