A Versatile Method for Inducible Protein Production in 293 Cells Using the PiggyBac Transposon System
Author:
Michael Iacovos P.
Reference22 articles.
1. Wurm FM (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 22:393–1398. https://doi.org/10.1038/nbt1026
2. Assenberg R, Wan PT, Geisse S, Mayr LM (2013) Advances in recombinant protein expression for use in pharmaceutical research. Curr Opin Struct Biol 23:393–402. https://doi.org/10.1016/j.sbi.2013.03.008
3. Schütz A, Bernhard F, Berrow N, Buyel JF, Ferreira-da-Silva F, Haustraete J, van den Heuvel J, Hoffmann J-E, de Marco A, Peleg Y et al (2023) A concise guide to choosing suitable gene expression systems for recombinant protein production. STAR Protoc 4:102572. https://doi.org/10.1016/j.xpro.2023.102572
4. Dyson MR (2016) Fundamentals of expression in mammalian cells. Adv Exp Med Biol 896:217–224. https://doi.org/10.1007/978-3-319-27216-0_14
5. Li Z, Michael IP, Zhou D, Nagy A, Rini JM (2013) Simple piggyBac transposon-based mammalian cell expression system for inducible protein production. Proc Natl Acad Sci USA 110:5004–5009. https://doi.org/10.1073/pnas.1218620110