Amplification of the PLAG-family genes—PLAGL1 and PLAGL2—is a key feature of the novel tumor type CNS embryonal tumor with PLAGL amplification
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Published:2022-11-27
Issue:1
Volume:145
Page:49-69
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ISSN:0001-6322
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Container-title:Acta Neuropathologica
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language:en
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Short-container-title:Acta Neuropathol
Author:
Keck Michaela-Kristina, Sill Martin, Wittmann Andrea, Joshi Piyush, Stichel Damian, Beck Pengbo, Okonechnikow Konstantin, Sievers Philipp, Wefers Annika K., Roncaroli Federico, Avula Shivaram, McCabe Martin G., Hayden James T., Wesseling Pieter, Øra Ingrid, Nistér Monica, Kranendonk Mariëtte E. G., Tops Bastiaan B. J., Zapotocky Michal, Zamecnik Josef, Vasiljevic Alexandre, Fenouil Tanguy, Meyronet David, von Hoff Katja, Schüller Ulrich, Loiseau Hugues, Figarella-Branger Dominique, Kramm Christof M., Sturm Dominik, Scheie David, Rauramaa Tuomas, Pesola Jouni, Gojo Johannes, Haberler Christine, Brandner Sebastian, Jacques Tom, Sexton Oates Alexandra, Saffery Richard, Koscielniak Ewa, Baker Suzanne J., Yip Stephen, Snuderl Matija, Ud Din Nasir, Samuel David, Schramm Kathrin, Blattner-Johnson Mirjam, Selt Florian, Ecker Jonas, Milde Till, von Deimling Andreas, Korshunov Andrey, Perry Arie, Pfister Stefan M., Sahm Felix, Solomon David A., Jones David T. W.ORCID
Abstract
AbstractPediatric central nervous system (CNS) tumors represent the most common cause of cancer-related death in children aged 0–14 years. They differ from their adult counterparts, showing extensive clinical and molecular heterogeneity as well as a challenging histopathological spectrum that often impairs accurate diagnosis. Here, we use DNA methylation-based CNS tumor classification in combination with copy number, RNA-seq, and ChIP-seq analysis to characterize a newly identified CNS tumor type. In addition, we report histology, patient characteristics, and survival data in this tumor type. We describe a biologically distinct pediatric CNS tumor type (n = 31 cases) that is characterized by focal high-level amplification and resultant overexpression of either PLAGL1 or PLAGL2, and an absence of recurrent genetic alterations characteristic of other pediatric CNS tumor types. Both genes act as transcription factors for a regulatory subset of imprinted genes (IGs), components of the Wnt/β-Catenin pathway, and the potential drug targets RET and CYP2W1, which are also specifically overexpressed in this tumor type. A derived PLAGL-specific gene expression signature indicates dysregulation of imprinting control and differentiation/development. These tumors occurred throughout the neuroaxis including the cerebral hemispheres, cerebellum, and brainstem, and were predominantly composed of primitive embryonal-like cells lacking robust expression of markers of glial or neuronal differentiation (e.g., GFAP, OLIG2, and synaptophysin). Tumors with PLAGL1 amplification were typically diagnosed during adolescence (median age 10.5 years), whereas those with PLAGL2 amplification were diagnosed during early childhood (median age 2 years). The 10-year overall survival was 66% for PLAGL1-amplified tumors, 25% for PLAGL2-amplified tumors, 18% for male patients, and 82% for female patients. In summary, we describe a new type of biologically distinct CNS tumor characterized by PLAGL1/2 amplification that occurs predominantly in infants and toddlers (PLAGL2) or adolescents (PLAGL1) which we consider best classified as a CNS embryonal tumor and which is associated with intermediate survival. The cell of origin and optimal treatment strategies remain to be defined.
Funder
Deutsche Kinderkrebsstiftung Everest Centre for Low-Grade Paediatric Brain Tumour Research Brain Tumour Charity Deutsches Krebsforschungszentrum (DKFZ)
Publisher
Springer Science and Business Media LLC
Subject
Cellular and Molecular Neuroscience,Neurology (clinical),Pathology and Forensic Medicine
Reference71 articles.
1. Central Nervous System Tumours (2021) WHO classification of tumours, 5th edition, volume 6. Edited by the WHO Classification of Tumours Editorial Board. 2021: International Agency for Research on Cancer 2. Abdollahi A (2007) LOT1 (ZAC1/PLAGL1) and its family members: mechanisms and functions. J Cell Physiol 210(1):16–25. https://doi.org/10.1002/jcp.20835 3. Abdollahi A, Pisarcik D, Roberts D, Weinstein J, Cairns P, Hamilton TC (2003) LOT1 (PLAGL1/ZAC1), the candidate tumor suppressor gene at chromosome 6q24-25, is epigenetically regulated in cancer. J Biol Chem 278(8):6041–6049. https://doi.org/10.1074/jbc.M210361200 4. Adnani L, Dixit R, Chen X, Balakrishnan A, Modi H, Touahri Y et al (2018) Plag1 and Plagl2 have overlapping and distinct functions in telencephalic development. Biol Open. https://doi.org/10.1242/bio.038661 5. Al Adhami H, Evano B, Le Digarcher A, Gueydan C, Dubois E, Parrinello H et al (2015) A systems-level approach to parental genomic imprinting: the imprinted gene network includes extracellular matrix genes and regulates cell cycle exit and differentiation. Genome Res 25(3):353–367. https://doi.org/10.1101/gr.175919.114
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