CYTOKINES AND PROTEINS OF THE EXTRACELLULAR MATRIX IN THE BLOOD OF RATS OF DIFFERENT AGES AFTER FILLING THE DEFECT IN THE FEMORAL METAPHYSIS WITH ALLOGENEIC BONE IMPLANTS SATURATED WITH MESENCHYMAL STEM CELLS

Abstract

Mesenchymal stem cells (MSCs) are used for the improvement of the repair of allografts. Aim. Based on the analysis of cytokines and proteins of the extracellular matrix in the blood serum of laboratory rats, to evaluate the course of metabolic processes after filling a defect in the femoral metaphysis with allogeneic bone implants saturated with MSCs. Material and Methods. On the model of a transcortical defect in the metaphysis of the femur, the content in the blood serum of 3 and 12 months white rats of interleukin-1 (IL-1), interleukin-6 (IL-6), transforming growth factor-b (TGF-b), osteocalcin (OC) and osteopontin (OP) on the 14th, 28th and 90 days was investigated. Results and Discussion. Use of MSCs in 3-month-old rats led to an increase in IL-1 concentration: 0.300(0.269; 0.329, 0.308(0.273; 0.339), 0.295(0.271; 0.319) pg/ml of IL-1 for the 14th, 28th and 90th days, respectively, which higher than the level of rats without MSCs by 1.69 (p=0.008), 1.37 (p=0.008) and 1.46 (p=0.008) times, respectively. For 12-month-old rats: IL-1 (0.414(0.387;0.437), 0.426(0.389; 0.448) and 0.407(0.368;0.423) pg/ml on the 14th, 28th and 90th days, respectively), which exceeded the data of rats without MSCs in 1.69 (p=0.008); 1.38 (p=0.008) and 1.46 times (p=0.008). In the blood serum of rats with MSCs TGF-b: 3-month: - 1.826(1.637;2.015), 1.379(1.247; 1.568) and 1.675(1.476;1.893) ng/ml on the 14th, 28th and 90th days, respectively, which was 1.57 (p=0.008), 1.98 (p=0.008) and 1.51 (p=0.008) times less than the data of the comparison group; 12-month-old - 1.976(1.749; 2.234), 1.492(1.218;1.734) and 1.813(1.636;2.028) ng/ml on the 14th, 28th and 90th days, respectively, which is less than the level of rats without MSCs of 1.50 ( p=0.008), 1.92 (p=0.008) and 1.51 (p=0.008) times. When introducing MSCs OP: 3-month-old rats - 13.55(12.90; 14.04), 14.14(13.43;14.87) and 14.12(13.75;14.77) ng/ml, which was higher than in rats without MSCs in 1.11 (p=0.008), 1.11 (p=0.008) and 1.17 (p=0.008) times; 12-month-old - 15.26(14.70; 15.81), 16.38(15.88; 16.91) and 16.06(15.53; 16.67) ng/ml, which is more than the comparison group 1.11 (p=0.008), 1.16 (p=0.008) and 1.22 (p=0.008) times; Conclusions. Saturation of allografts with MSCs led to an increase in biochemical markers of inflammation from the 14th day. Bone remodeling in rats with MSCs was characterized by lower activity. In 12-month-old rats in comparison with 3-month-old animals we noted more active inflammation, with a slowed activity in bone remodeling.