Influence of ozone generated by pulsed alternating electric current on the viability of representatives of oral microflora involved in the development of prosthetic stomatitis

Abstract

Topicality. When using removable dentures, in 84.26±1.19 % of cases patients have various changes in the mucous membrane of the prosthetic bed with pronounced changes in microbial ecology which are exacerbated by poor oral hygiene and removable dentures. Ozone therapy is a new and promising area of treatment of microbial lesions of the oral mucosa. It has antiseptic, anti-inflammatory, analgesic, immunomodulatory effects and promotes activation of intracellular metabolism, improvement of rheological properties and microcirculation of blood; it also strengthens reparative ability of the injured tissues. The aim of the study is to evaluate the in vitro effect of ozone generated by the device for use in dental practice on the survival of the representatives of aerobic and facultative anaerobic microflora of the oral cavity, which are important in the development of prosthetic stomatitis. Materials and methods of research. In vitro experiments helped to study the effect of ozone generated by pulsed alternating electric current (Ozone DTA device for use in dental practice) on the survival of the representatives of aerobic and facultative anaerobic microflora of the oral cavity, which may be potential factors in inflammatory processes in prosthetic bed tissues and the development of prosthetic stomatitis. The results were processed by the method of variation statistics and two-way analysis of variance ANOVA (MS Excel 2003). Results. A direct dependence of the influence of the survival level of S. aureus in suspension on the ozone generation capacity and the time of exposure to ozone was established. Different types of oral microorganisms differ in their sensitivity to ozone. Cultures of S. epidermidis, β-hemolytic Streptococcus group G, α-hemolytic streptococci S. sanguinis and S. oralis, yeast-like fungi Candida tropicalis are characterized by relatively higher sensitivity to the generated ozone. Conclusion. The obtained experimental results microbiologically substantiate the possibility and expediency of using ozone therapy to prevent the occurrence and treatment of prosthetic stomatitis. Based on the performed experiments, the optimal mode of ozone therapy with the Ozone DTA device was established to ensure the antimicrobial effect at the level of 98.8–100%. Key words: ozone, microorganisms, antimicrobial action, oral cavity.