A PRELIMINARY STUDY ON CYTOCHROME 2E1 GENE SINGLE NUCLEOTIDE POLYMORPHISMS AMONG ETHNIC FULANI IN NORTH WESTERN NIGERIA

Abstract

Single nucleotide polymorphisms of xenobiotics metabolizing enzymes are critical in inter-individual variability in drug response. Cytochrome P450 2E1, which is highly polymorphic belongs to these enzymes and ethnicity signicantly determine their expressions. Volunteers from Fulani extractions were recruited through their expressed consents. Five mls of blood sample was collected in EDTA container used for DNA extraction, PCR and sequencing. The consensus sequence generated for individual participants were located to the Cyp 2E1 gene and spanned both target rs 2031920 to rs 3813867 polymorphism region. Chromatograms were validated with Bioedit software. Three (3) volunteers (15%) revealed new rs 147346389 A>C polymorphism at nucleotide position 4029. Also, three (3) other volunteers (15%) revealed rs 35806299 A>G polymorphism at position 3661. One (5%) new (unlabelled) A>C polymorphism was revealed at position 3906.