IMMUNOHISTOCHEMICAL EXPRESSION OF C-KIT AND S-100 MARKERS IN URETEROPELVIC JUNCTION OBSTRUCTION AND ITS CORRELATION WITH HISTOPATHOLOGY AND MASSON'S TRICHROME STAINING
-
Published:2022-10-01
Issue:
Volume:
Page:25-27
-
ISSN:
-
Container-title:INDIAN JOURNAL OF APPLIED RESEARCH
-
language:en
-
Short-container-title:IJAR
Author:
D Chaitra.1, Padmaja G. J. Vani2, Geetha Kayla3, Vedire Yeshwanth4
Affiliation:
1. Senior Resident, Department of Pathology, Osmania Medical college, Hyderabad. 2. Professor &HOD, Department of Pathology, Osmania Medical college, Hyderabad. 3. Associate Professor, Department of Pathology, Osmania Medical college, Hyderabad. 4. Tutor, Osmania Medical college, Hyderabad.
Abstract
Background: Congenital obstructive nephropathy is the principal cause of end stage renal disease (ESRD) in children (
Beneldet al. 2003). Obstructive nephropathy leads to Hydronephrosis, dened clinically by an enlargement of the
kidney as a result of urine accumulation in the renal pelvis or calyces. [1,2] Ureteropelvic junction (UPJ) obstruction is the most common cause
of hydronephrosis with an estimated incidence of 1 in 1000–1500 newborns ( Changet al. 2004). [2,3] Aims: To evaluate the pathogenesis of
congenital ureteropelvic junction obstruction (UPJO) by histopathology, IHC markers CD117 & S100 and special stain Masson's Trichrome.
Methods And Materials: The study group consists of 50 Pediatric cases presenting with intrinsic ureteropelvic junction obstruction and 15
patients with Wilms tumor with no UPJO formed the control group. All the cases and controls were subjected to histopathological examination,
immunohistochemical staining with markers CD117 & S100 and special stain Masson's Trichrome. Mann – Whitney U test and Pearson's chisquare test were used for statistical analysis. The cases had reduce Results: d CD117 and S100 expression, Irregularity of muscle bers, increased
wall thickness & muscle thickness and submucosal collagen deposition compared to controls. Decreased ICC interpreted by Conclusions:
decreased CD117, decreased neural innervation interpreted by decreased S100, structural changes - Irregularity of muscle bers arrangement,
increased submucosal collagen deposition and increased smooth muscle thickness possibly cause disruption of the mobility of UPJ and lead
functional obstruction.
Publisher
World Wide Journals
Subject
Applied Mathematics,Atmospheric Science,Computers in Earth Sciences,General Environmental Science,Safety Research,Law,Literature and Literary Theory,Linguistics and Language,Language and Linguistics,Cultural Studies,History and Philosophy of Science,Multidisciplinary,General Engineering,Religious studies,Education,Social Psychology,Economics, Econometrics and Finance (miscellaneous),Public Administration,Sociology and Political Science,Sociology and Political Science,Sociology and Political Science,Social Sciences (miscellaneous),Insect Science,Soil Science,Microbiology
Reference26 articles.
1. Benfield M.R., McDonald R.A., Bartosh S., Ho P.L., Harmon W. (2003) Changing trends in pediatric transplantation: 2001 Annual Report of the North American Pediatric Renal Transplant CooperativeStudy. Pediatr. Transplant. 7, 321–335. 2. Klein, J., Gonzalez, J., Miravete, M., Caubet, C., Chaaya, R., Decramer, S., Bandin, F., Bascands, J. L., Buffin-Meyer, B., & Schanstra, J. P. (2011). Congenital ureteropelvic junction obstruction: human disease and animal models. International journal of experimental pathology, 92(3), 168–192. https://doi.org/10.1111/j.1365-2613.2010.00727.x 3. Chang C.P., McDill B.W., Neilson J.R. et al. (2004) Calcineurin is required in urinary tract mesenchyme for the development of the pyeloureteral peristaltic machinery. J. Clin. Invest. 113, 1051–1058. 4. Hashim Hashim, Christoper R. J. Woodhouse EAU(2012) Ureteropelvic Junction Obstruction European Urology Supplements 11 (2012) 25-32 https://doi.org/10.1016/j.eursup.2012.01.004 5. Carr MC, El-Ghoneimi A (2007) Anomalies and surgery of the ureteropelvic junction. In: Campbell-Walsh urology,Section XVII: pediatric urology, 9th edn. Saunders Elsevier,pp 3359–3382
|
|