CLINICO-HEMATOLOGICAL STUDY OF ACUTE LYMPHOBLASTIC LEUKEMIA AND THEIR CORRELATION WITH INFLAMMATORY MARKERS IN SERUM.-Reference-Cited by-同舟云学术

CLINICO-HEMATOLOGICAL STUDY OF ACUTE LYMPHOBLASTIC LEUKEMIA AND THEIR CORRELATION WITH INFLAMMATORY MARKERS IN SERUM.

Published:2021-12-15 Issue: Volume: Page:6-12
ISSN:
Container-title:GLOBAL JOURNAL FOR RESEARCH ANALYSIS
language:en
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Author:

Sharma Kavyanjali¹,Usha Usha²,Tilak Vijai³,Gupta Vineeta⁴,Rai Madhukar⁵,Narayan Gopeshwar⁶,Rani Deepa⁷

Affiliation:

1. M.Sc., Ph.D. Scholar, Department of Pathology, Institute of Medical Sciences, BHU, Varanasi - 221005, Uttar Pradesh, India.

2. MD, MNAMS, FICN, FICAI, Ex-Professor, Department of Pathology, Institute of Medical Sciences, BHU, Varanasi - 221005, Uttar Pradesh, India.

3. MD, Professor & Head, Department of Pathology, Institute of Medical Sciences, BHU, Varanasi - 221005, Uttar Pradesh, India.

4. MD, DNB, MAMS, FRCPCH, FUICC, Professor, Department of Pediatrics. Sir Sundarlal Hospital, BHU, Varanasi - 221005, Uttar Pradesh, India.

5. MD, DM, Professor, Department of Medicine, Institute of Medical Sciences, BHU, Varanasi - 221005, Uttar Pradesh, India.

6. Ph.D., Professor, Department of Molecular & Human Genetics, Institute of Science, BHU, Varanasi - 221005, Uttar Pradesh, India

7. MD, Associate Professor, Department of Pathology, Institute of Medical Sciences, BHU, Varanasi - 221005, Uttar Pradesh, India.

Abstract

SUMMARY: Acute lymphoblastic leukemia (ALL) is early childhood hematological malignancies. In present scenario immunophenotyping became an important tool for subtyping of ALL into B-ALL and TALL. In order to understand the mechanism of development of leukemia it is important to study the cytokine environment of malignant cells. OBJECTIVE: Aim of the present study was to evaluate clinical and hematological features in ALL and correlate serum levels of IL6 and IL-10 expression in ALL patients and their subtypes. MATERIALS & METHODS: A total of 68 ALL cases along with 20 healthy controls were included in the study between periods of 2015 to 2017. About 4 mL blood samples were collected from all cases for immunophenotyping and serum studies. Levels of IL6 and IL10 were determined in all cases by ELISA. RESULT: In the present study immunphenotyping was done in all cases of ALL, which showed 52 cases (76.5%) of B-ALL and 16 cases (23.5%) of T-ALL. T-ALL was mostly found in higher aged children than B-ALL. A male predominance was seen in all cases. No signicant differences in hemoglobin levels and platelet counts were found between T-ALL and B-ALL. A signicantly high percentage of T-ALL cases were having more than 50000 cells per microliter than B-ALL (56.2% vs. 23.1%). Almost similar clinical features were found in both subgroups, only bleeding manifestation was found signicantly higher in T-ALL than B-ALL (31.2% vs.11.5%). Acute lymphoblastic leukemia (ALL) patients were associated with signicantly elevated serum IL6 and IL10 level than the healthy control group. Mean levels of serum IL6 were 167.9±306.46 pg/mL in ALL, and 6.51 ± 2.27 pg/mL in healthy control group. Mean IL10 levels were 70.56±111.48 pg/mL in ALL and 29.39 ± 4.27 pg/mL in control group. There were no signicant differences found in IL-6 and IL-10 serum levels between T-ALL and B-ALL. CONCLUSION: Present study found elevated level of IL-6 and IL-10 in ALL patients which suggest possible role of these cytokines in disease transformation. Detection of IL-6 and IL-10 in newly diagnosed patient may predict disease outcome and possibly poor prognosis in patients

Publisher

World Wide Journals

Reference32 articles.

1. Katz AJ, Chia VM, Schoonen WM, Kelsh MA. Acute lymphoblastic leukemia: an assessment of international incidence, survival, and disease burden. Cancer Causes Control. 2015 Nov 1;26(11):1627–42.

2. MC B, T N, P B, B B, H B, W K, et al. Immunophenotyping of acute leukemia and lymphoproliferative disorders: a consensus proposal of the European LeukemiaNet Work Package 10. Leukemia. 2011;25(4):567–74.

3. Anand M, Chodda SK, Parikh PM, Nadkarni JS. Abnormal levels of proinflammatory cytokines in serum and monocyte cultures from patients with chronic myeloid leukemia in different stages, and their role in prognosis. Hematol Oncol. 1998;16(4):143–54.

4. Burger R. Impact of Interleukin-6 in Hematological Malignancies. Transfus Med Hemotherapy. 2013 Oct;40(5):336.

5. Drabko K, Bojarska-Junak A, Kowalczyk JR. Clinical immunology
Serum concentration of IL-2, IL-4, IL-10 and TNF-α in children with acute lymphoblastic leukemia – possible role of oxidative stress. Cent Eur J of Immunology. 2008;33(3):146–9.