| 中空高分子荧光微球的制备及其免疫层析检测应用 |
| Preparation of hollow polymer fluorescent microspheres and its application in lateral flow immunoassay |
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| 摘要: 以过硫酸钾（KPS）为引发剂，甲基丙烯酸缩水甘油酯（GMA）和苯乙烯（St）为单体，采用无皂乳液聚合法制备了表面官能团化的高分子微球。研究发现添加微量NaCl制备得到的高分子微球，经四氢呋喃（THF）溶胀处理后，能够得到单分散且表面完整的中空P （St-co-GMA）微球。随后使用有机溶胀的方式将Eu（Ⅲ）配合物染料负载于微球壳层中，实现了α1微球蛋白（α1-MG）和β2微球蛋白（β2-MG）的免疫层析检测。 |
| 关键词: Eu（Ⅲ）配合物 甲基丙烯酸缩水甘油酯 苯乙烯 中空微球 免疫层析 |
| 基金项目: 国家重点研发计划“基础科研条件与重大科学仪器设备研发”专项资助项目基金（No.2022YFF0710000）资助。 |
| Abstract: The functionalized polymer microspheres were prepared by soap-free emulsion polymerization using potassium persulfate (KPS) as the initiator, glycidyl methacrylate (GMA) and styrene (St) as monomers. It was found that microspheres prepared by adding a trace amount of NaCl could be swelled by tetrahydrofuran (THF) to obtain monodisperse hollow microspheres with complete surfaces. The Eu（Ⅲ） complex dye was then loaded into the microspheres shell by means of organic swelling, and immunochromatographic detection of α1 microglobulin (α1-MG) and β2 microglobulin (β2-MG) was performed. |
| Keywords: Eu（Ⅲ） complex glycidyl methacrylate styrene hollow microspheres lateral flow immunoassay |
| 投稿时间：2023-07-11 修订日期：2023-09-19 |
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| 洪正道,李振华,徐明,冯玮,李富友.中空高分子荧光微球的制备及其免疫层析检测应用[J].无机化学学报,2023,39(11):2055-2064. |
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| Support information: 相关附件： 20230261_supporting_information.doc |
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